The ViriChip™ Virus Detection Platform
| Problem |
Solution |
Technology
| The 4x4mm ViriChipTM is functionalized with an ultramicro array of antibodies using BioForce's Nano eNablerTM |
| The ViriChipTM captures intact viruses. The specificity of the captured particles is determined by the specificity of the antibodies. |
| After less than two hours of incubation, virus particles are directly detected using an Atomic Force Microscopy-based reader. The detection is non-destructive and very sensitive, and can also provide size and shape information about the captured particles. |
Advantages
Direct detection | Multiplexing |
Detects actual viruses, not their byproducts | Multiple virus detection in a single test or increased speed for single type deposition |
Fast | Label-free |
Incubation and detection take 30 minutes to 2 hours | Does not require labels |
Non-destructive | No sample preparation |
Leaves viruses bioactive | Detects viruses directly from blood, serum, water and other raw samples |
Sensitive | Portable |
Detects viruses even from minute concentrations | Portable AFMs are already available and BioForce is working on a portable ViriChipTM Reader |
References
Lynch, M.; Mosher, C.; Huff, J.; Nettikadan, S.; Johnson, J. and E. Henderson: Functional protein nanoarrays for biomarker profiling. Proteomics 4: 1695-1702, 2004.
Nettikadan, S.; Johnson, J.; Vengasandra, S.G.; Muys, J. and E. Henderson: ViriChip: a solid phase assay for detection and identification of viruses by atomic force microscopy. Nanotechnology 15: 383-389, 2004.
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