The ViriChip™ Virus Detection Platform

Problem
While the last SARS outbreak did cost the global economy $11 BN, a potential Avian Flu pandemia can cost $800 BN and millions of lives. The 4.5 million US cases of Hepatitis C costs the US healthcare $9 BN annually. There are 40 million worldwide cases of HIV and it continues to spread, partially due to lack of reliable detection.

Solution
BioForce Nanosciences developed a platform for fast, sensitive and non-destructive virus detection. The platform consists of a silicon chip functionalized with an ultramicro array of antibodies using the Nano eNablerTM, and a detection system based on Atomic Force Microscopy.

Technology

The 4x4mm ViriChipTM is functionalized with an ultramicro array of antibodies using BioForce's Nano eNablerTM

The ViriChipTM captures intact viruses. The specificity of the captured particles is determined by the specificity of the antibodies.

After less than two hours of incubation, virus particles are directly detected using an Atomic Force Microscopy-based reader. The detection is non-destructive and very sensitive, and can also provide size and shape information about the captured particles.

Advantages

 

Direct detection

Multiplexing

Detects actual viruses, not their byproducts
Minimizes false positives

Multiple virus detection in a single test or increased speed for single type deposition

Fast

Label-free

Incubation and detection take 30 minutes to 2 hours

Does not require labels

Non-destructive

No sample preparation

Leaves viruses bioactive
Increases subsequent PCR sensitivity 10,000-fold

Detects viruses directly from blood, serum, water and other raw samples

Sensitive

Portable

Detects viruses even from minute concentrations

Portable AFMs are already available and BioForce is working on a portable ViriChipTM Reader

References

 

 

Lynch, M.; Mosher, C.; Huff, J.; Nettikadan, S.; Johnson, J. and E. Henderson: Functional protein nanoarrays for biomarker profiling. Proteomics 4: 1695-1702, 2004.
Nettikadan, S.; Johnson, J.; Vengasandra, S.G.; Muys, J. and E. Henderson: ViriChip: a solid phase assay for detection and identification of viruses by atomic force microscopy. Nanotechnology 15: 383-389, 2004.

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